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Research Associate
3 months ago
Research Associate / Scientist - Protein Science
REED are working with an exciting life sciences start-up based in the Cambridge area to fill a vacancy within their protein engineering, expression, purification and characterisation team.
The company have already received a huge investment and are set upwith enough money for at least the next 4 years of operation.
In this role you will
- Perform DNA cloning and prepare DNA constructs for transfections
- Express novel TCR formats in HEK293 and CHO cells in small, medium and large scale
- Perform protein purification and ensure proteins are of highquality using protein analytical and assay techniques (SDS-PAGE, HPLC-SEC, ELISA)
- Maintain and establish cell lines required for protein production
- Provide highquality protein material to the downstream protein characterisation team
- Support protein team in functional and biophysical characterisation
- Analyse and present work/data in group meetings and produce accurate scientific reports and SOPs
- Maintain uptodate and accurate laboratory record of all experimental activities
About you
- Handson experience in protein production in mammalian cells such as proteinbased biologics (e.g. antibodies or other biologics) and protein purification
- Experience with protein expression in E. coli and troubleshooting difficult expression would be highly desirable
- Practical knowledge of the AKTA purification platform and using conventional chromatography resins e.g. ion exchange
- Good working knowledge of a range of methodologies to characterise proteins
- Mammalian cell culture aseptically culturing and maintaining cell lines, transfections for protein production
- Knowledge and/or experience in antibody or soluble TCR development would be highly desirable
You could really make your mark within supportive and diverse work environment. You will be encouraged to improve your skills and grow along with the company.
If this looks of interest and you meet the requirements, don't miss this opportunity
