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Postdoctoral Research Associate in Membrane

4 months ago

Harwell, United Kingdom Springer Nature Full time

**Postdoctoral Research Associate in Membrane Proteomics**:

- Employer- The Rosalind Franklin Institute- Location- Harwell, Didcot- Salary- £35,500-£43,500 per annum- Closing date- 16 Apr 2024- Discipline
Biomedicine, Chemistry
Job Type
Postdoctoral
Employment - Hours
Full time
Duration
Fixed term
Qualification
PhD
Sector
Charity/NGO- You need to sign in or create an account to a job.
- Job Details
- Company

**Job Details**:
The Rosalind Franklin Institute is a national research centre, funded by the UK government through UK Research and Innovation, dedicated to bringing about transformative changes in life science through interdisciplinary research and technology. Recently, we were awarded a Wellcome Trust Discovery Award to investigate the nature of the Gram-negative bacteria outer membrane. As part of this project, we are currently looking for a science driven, but community oriented Postdoctoral Research Associate to join our proteomics group.

One of the major goals of the role is to improve technologies for the characterisation of membrane proteins initially in terms of discovering the complete protein inventory (including copy numbers) but then rapidly moving towards studying specific protein complex formation and the role of their lipid environment. The PDRA will be charged with creating maps of the membrane proteome for a number of gram-negative bacteria including E. Coli. Furthermore, protein interfaces both to proteins and lipids will be characterised through crosslinking approaches. The PDRA will be embedded within a larger consortium spanning multiple disciplines and institutes including Oxford University and UCL.

We are looking for someone with excellent skills in the following areas:

- Practical experience with several mass spectrometer types including orbitraps.
- Practical experience with several fragmentation approaches utilised in the field of biomolecular mass spectrometry.
- Ability to carry out analytical procedures that are applied for proteome, lipidome and crosslinked protein characterisation to a high degree of precision.
- Ability to analyse quantitative proteomic data with a degree of statistical rigour using common freely available software packages.
- Familiarity with a wide range of chromatographies employed in proteomics and lipidomics.
- Practical experience with hyphenating LCs to mass spectrometers.
- Experience with protein chemistry and standard bioanalytical approaches.
- Practical experience with proteomics mass spectrometry.

**Responsibilities**

The main specific responsibilities of this role will include:

- Hyphenate LCs to a range of state-of-the-art mass spectrometers.
- Characterise a range of protein, lipid and proteolipid samples using a range of mass spectrometers and fragmentation regimes.
- Carry out analytical procedures that are applied for protein crosslinking characterisation to a high degree of precision including sample preparation.
- Analyse quantitative proteomic data with a degree of statistical rigour using common freely available software packages.
- Help to devise then create proteomic methodologies to address protein-protein and protein-lipid complexes.
- Carry out collaborative projects with colleagues and other research groups.
- Act as a source of information and advice to other members of the group on scientific protocols and experimental techniques.
- Contribute to the public engagement in science programme of the Franklin.

**In return we offer**:

- 25 days holidays plus bank holidays and Christmas holiday shutdown
- Generous pension scheme (employer’s contribution currently up to 18%)
- Group Life Assurance (also known as Group Life Insurance or Death in Service)
- Hub building with state-of-the-art laboratories
- Training and development opportunities for staff at all levels
- Bus pass discount scheme and good transport links to Oxford and surrounding areas
- Cycle to Work Scheme
- Access to employee discount platform (Perkbox)
- Occupational Health and wellbeing support including Employee Assistance (24/7 support and counselling)
- Health Cash Plan
- Subsidised canteen and food outlets on campus
- Free on-site parking
- Campus location in beautiful countryside with social and sports clubs open to staff

About us: The Rosalind Franklin Institute is a new national Research Centre, funded by the UK government through UK Research and Innovation, dedicated to bringing about transformative changes in life science through interdisciplinary research and technology.

Location: The Franklin is based in Harwell Campus, UK’s innovation hub and well-established as one of the leading science and innovation campuses in Europe.

The Franklin’s underlying aim is to produce the best science for research today, and this means resolutely embracing a diverse team, who have a wide range of experiences, skills and knowledge to push forward on the innovative work our institution delivers. Both our work and our institution are better f