Molecular Plant Pathologist

4 weeks ago

Cambridge, Cambridgeshire, United Kingdom National Institute of Agricultural Botany (NIAB) Full time £27,562
About the Role

We are seeking a highly skilled Molecular Plant Pathologist to join our team at the National Institute of Agricultural Botany (NIAB). As a key member of our Plant Pathology department, you will play a crucial role in advancing our understanding of plant pathogens and their interactions with crops.

As a Molecular Plant Pathologist, you will be responsible for conducting molecular biology and microbiology research to support projects involving fungicide sensitivity testing, plant pathogenicity testing, genotyping, and functional genetics. You will work closely with our team of experts to develop and implement new research techniques and methodologies to improve crop protection and food security.

The ideal candidate will have a strong background in molecular biology, microbiology, and plant pathology, with experience in DNA and RNA extractions, PCR, cloning, and aseptic technique. You will also have excellent organizational skills, attention to detail, and the ability to work independently and as part of a team.

We offer a competitive salary and benefits package, as well as opportunities for professional development and growth. If you are passionate about advancing crop science and improving food security, we encourage you to apply for this exciting opportunity.

Key Responsibilities
  • Fungicide resistance testing: isolation of plant pathogenic fungi, maintaining isolate collections, sensitivity bioassays and data analysis
  • Plant pathogen genotyping: DNA extractions, PCR, preparation of samples for sequencing (Sanger or NGS), sequence data analysis, molecular diagnostics such as qPCR
  • Assisting with research aimed at characterisation of virulence effectors and fungicide-resistant alleles in cereal-infecting fungi: development of various DNA constructs, viral (VIGS/VOX) and fungal transformations, qRT-PCR, validation of protein-protein interactions
  • Assistance with other projects requiring molecular biology support; general laboratory support, e.g. ensuring laboratory equipment and consumable stock levels are maintained
Requirements
  • A BSc degree in a relevant subject (such as biological sciences, plant sciences, microbiology or genetics) or equivalent qualifications/experience
  • Experience in molecular biology: DNA & RNA extractions, PCR, cloning
  • Experience in aseptic technique and general microbiology methods
  • Organisational skills to keep samples, data files and laboratory notes in good order
  • Good IT skills, such as Microsoft Office, and the ability to learn to use new software packages associated with lab machines
  • Basic data-processing and analysis skills: dataset compilation in Microsoft Excel; basic statistical analyses; DNA sequence analysis (e.g. using Geneious)
  • Accuracy and attention to detail in following experimental protocols
  • A collaborative approach and excellent communication skills to work across different groups within a department
  • Self-motivation and initiative to plan your own day-to-day work and manage laboratory matters such as consumables stock levels and equipment repairs
Desirable Requirements
  • Experience isolating and culturing fungi and/or plant pathogens
  • Experience working with plants in a glasshouse or growth chambers
  • Background knowledge of plant pathogens and crop protection
  • Basic bioinformatic analysis skills, e.g. BLAST, gene function analyses and effector identification software
  • Experience with high throughput sequencing (e.g. Illumina, Nanopore) or molecular diagnostics (e.g. qPCR, RT-qPCR)
  • Experience in fungal, microbial, viral or plant transformations
  • Flexibility to work alone or as part of a small team
  • Commitment to your own personal development and willingness to learn new skills
  • A driving licence or access to independent means of transport

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