Senior Scientist
3 weeks ago
Job Title: Senior Scientist – Global Biopharmaceutical
Contract: 12 month contract
Location: Slough
Salary: £28.09 - £33.58 PAYE
SRG are working with a Global Biopharmaceutical company to help them find a Senior Scientist to join their busy team. This is an exciting opportunity for an individual experienced in protein analysis.
You will be part of the Protein Production Group playing a key role in project success by generating high quality protein reagents in a timely manner. You will also assist with antibody discovery.
The successful candidate will have the opportunity to rapidly impact on a wide range of our NBE & NCE projects, and to significantly contribute to the progression of these projects into the clinic and beyond.
Key Responsibilities will include:
- You will be responsible for expressing and purifying high-quality recombinant protein reagents to support UCB’s novel world-leading antibody and NCE drug discovery platforms for a variety of exciting therapeutic projects.
- You will have an excellent and proven track record of protein reagent delivery.
- You will use your excellent practical skills, communication and team working abilities to drive innovative projects forward from the laboratory.
- You will be responsible for clearly presenting work and engaging in discussion in a variety of cross-functional and external collaborator meetings.
Candidate Requirements:
- will have extensive experience (PhD holder or graduate with 4-5 years) in protein biochemistry, focusing on recombinant protein production.
- Biology (DNA manipulation techniques and comprehensive knowledge and experience with software used in manipulating protein and DNA sequences (VNTI) and molecular visualisation of proteins (PyMOL or similar).
- in E.coli, insect and mammalian cell expression systems (and experience in 15N, 13C and 2H labelled expression in E.coli)
- independent in protein purification techniques (affinity, ion exchange, hydrophobic interaction chromatography, size exclusion chromatography, analytical SEC, UV-Vis spectroscopy, and protein refolding techniques) and extensive experience working with AKTA purification platforms and associated software.
- competent in protein characterisation techniques: SDS-PAGE, Western blot, analytical SEC, UV-Vis spectroscopy and UPLC.
- biochemistry knowledge with hands on experience of protein labelling and characterisation.
- understanding of recombinant protein expression systems and the molecular biology involved in generating expression constructs.
- Thorough knowledge of the latest technologies involved in protein purification.
- Track record of recombinant protein production covering multiple protein classes including enzymes, structural proteins, cell signalling factors, cell surface receptors and their ligands produced as intracellular and secreted proteins.
- Ability to work on several projects and conduct experiments in parallel.
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