Research Assistant

Previous experience in ES-cell culture and gene targeting in ES cells is an advantage but not essential. Expertise in standard molecular biology techniques (PCR, sequencing, or _in-vitro_ transcription) are also desirable. Training in all skills including dissection will be provided.

**Main duties / key responsibilities**:

- ES-cell culture and manipulation
- Extraction of mouse tissues
- Characterisation and quality control of genetically modified materials employing PCR- and ddPCR-based techniques and nanopore sequencing
- Genotyping
- Gene expression studies
- Sample management and communication of results.
- Other general duties required are to ensure the smooth running of a Molecular Biology laboratory i.e., ordering reagents - tasks shared with rest of the team

**Working relationships**:

- Process samples/tasks by working as a team with the other Molecular and Cellular Biology (MCB) team members, as directed by the MCB Deputy Head initially or occasionally by the Head of the Genotyping Core
- Work with scientists in the different research groups, to help process samples as efficiently as possible

**Additional information**:
Some flexible working patterns and paid weekend work may be required at the discretion of the supervisor.

**Equality & Diversity**:
The MRC values the diverse skills and experience of its employees and is committed to achieving equality of treatment for all. Our objectives are that all individuals shall have equal opportunities for employment and advancement on the basis of their skills, aptitudes and abilities. The MRC is committed to the engagement and retention of the best possible talent and to creating an environment that encourages excellence in scientific research through good equality and diversity leadership and management.

**Corporate/Local responsibilities & requirements**:
The post holder must at all times carry out their responsibilities with due regard to the MRC’s:

- Code of Conduct
- Equality and Diversity policy
- Health and Safety policy
- Data Protection and Security policy

Job descriptions should be reviewed on a regular basis and at the annual appraisal. Any changes should be made and agreed between the post holder and their manager.

The above lists are not exhaustive and the post holder is required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, cooperatively and flexibly in line with the requirements of the post and the MRC.
- Person Specification**Education / Qualifications / Training required**:

- Essential:_
- An understanding of basic techniques (qPCR, cloning, aseptic technique)
- Desirable:_
- An understanding of genetic modification in laboratory animals.
- MSc in Biological Sciences with several years’ laboratory-based molecular biology training
- An understanding of genome-engineering techniques (gene targeting, CRISPR-based tools)

**Previous work experience required**:

- Essential: _
- Previous work in aseptic conditions and/or using PCR-based techniques
- Desirable: _
- Previous work with genetically modified materials
- Work with ES cells or digital PCR
- Previous experience in genotyping would be desirable, although some training in molecular techniques and automated liquid handling can be give
- Evidence of quality and customer-focused work

**Knowledge and experience**:

- Essential:_
- Experience in one of the following Molecular and Cellular Biology techniques: ES-cell culture and gene targeting, PCR, Sanger sequencing analysis, ddPCR, _in vitro _transcription
- Computer literacy is essential for the recording and communication of results
- Desirable:_
- Working and adhering to standard operating procedures, and experience of process analysis and refinement
- Experience in service provision

**Personal skills/behaviours/qualities**:

- Essential: _
- Organised, hard-working with a strong eye for detail and able to work well as part of a team
- Desirable: _
- Able to communicate clearly and concisely with a variety of scientists at all levels (from principal investigators to research assistants and animal technicians)