Research Assistant/associate in Microbiology or

2 weeks ago

Newcastle upon Tyne, United Kingdom Newcastle University Full time

**Salary**:
**Research Assistant**: £31,396 to £32,982 per annum
**Research Associate**: £33,966 to £44,263 per annum

Newcastle University is a great place to work, with excellent benefits. We have a generous holiday package; plus the opportunity to buy more, great pension schemes and a number of health and wellbeing initiatives to support you.

**Closing Date**: 23 June 2024

The Role

We are looking to appoint a talented and highly motivated Research Assistant/Associate with strong background in microbiology and/or biochemistry. You will work within the Faculty of Medical Sciences, Biosciences Institute, under the guidance of Prof. Bert van den Berg.

The project is funded by the BBSRC and will focus on understanding the biogenesis of outer membrane (OM) protein complexes within the Bacteroidetes phylum. The Bacteroidetes are a large Gram-negative bacterial phylum that is widely distributed within the environment (soil, sea water) and in animal microbiomes, including that of the human gut. In contrast to model Proteobacteria such as E. coli, Bacteroidetes are characterised by having large numbers of OM protein complexes that consist of integral beta-barrel proteins and surface exposed OM lipoproteins. The project is based on our exciting recent discovery that the Bacteroidetes contain a distinct BAM (beta-barrel assembly machinery) complex that we hypothesise mediates the folding and assembly of OM protein complexes. Based on preliminary structural analysis, the Bacteroidetes BAM complex is very different from that of Proteobacteria and likely has evolved additional functions. Therefore, this project will add a completely new chapter to the book of OM biogenesis.

This post is fixed term for a period of 36 months.
- Proficient wet lab skills in molecular biology and protein biochemistry
- Experience in experimental structural biology, preferably cryo-electron microscopy
- Knowledge of membrane protein structure and function
- Knowledge of biomolecular visualisation software like ChimeraX and Pymol

Please note that if you are successful to this role, you will require medical clearance before you can commence in the role.

As part of our commitment to career development for research colleagues, the University has developed 3 levels of research role profiles. These profiles set out firstly the generic competences and responsibilities expected of role holders at each level and secondly the general qualifications and experiences needed for entry at a particular level.

Key Accountabilities
Although working under the general guidance of an academic or Principal Investigator, the postholder will contribute ideas, including enhancements to the technical or methodological aspects of their studies, thus providing substantial 'added value'
Develop and carry out the specified project using appropriate techniques and equipment as outlined in the personal requirements
Determine appropriate methodologies for research, with advice and support where required
Begin to write, with appropriate support, proposals for individual research funding or, where funders do not permit this, contribute to the writing of collective bids
Assess research findings for the need/scope for further investigations
Contribute to the writing up of their research for publication and dissemination, either through seminar and conference presentations or through publications
Present research findings, either at conferences or through publications in reputable outlets appropriate to the discipline
May be involved in the supervision, with guidance, of final year undergraduate research projects and in providing support to postgraduate research students or Research Assistants
Will need to work with the support staff and, on occasions, with undergraduate and postgraduate students, and interact intellectually with other academic members of the Institute
May contribute to events celebrating the public engagement of science/social sciences/humanities
Develop an awareness of University structures, policies and procedures and relevant issues in the higher education, research, social and political environment

The Person

Knowledge, Skills and Experience
Ability to work well as part of a team and rapidly acquire new skills
Likelihood of advanced skills directly related to the research projects
High level of analytical and problem-solving capability
Ability to communicate complex information with clarity and to encourage the commitment of others
Experience of research with clear transferable skills and some experience or awareness of the research environment
Presentations at conferences and/or high-quality publications
Strong practical skills in molecular biology and protein biochemistry
Desirable
Experience in experimental structural biology such as X-ray crystallography and/or cryo-electron microscopy
Experience in membrane protein expression and purification
Experience in culturing anaerobic bacteria
Knowledge of outer membrane protein structure and fun

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