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Research Assistant- Microbiology
3 months ago
**Salary**: £31,396 to £32,332 per annum
Newcastle University is a great place to work, with excellent benefits. We have a generous holiday package; plus the opportunity to buy more, great pension schemes and a number of health and wellbeing initiatives to support you.
**Closing Date**: 12 May 2024
The Role
We are seeking a highly motivated Research Assistant to join our research team. You will support our team in using systems microbiology approaches to dissect Gram-negative envelope biology.
Much of the ecological success of bacteria can be attributed to their robust, protective envelope. The cell envelope is a complex structure, featuring a mesh-like peptidoglycan (PG) layer that provides mechanical protection to the cell. In Gram-negative bacteria, this PG mesh lays between two lipid bilayers, named the inner and the outer membrane. During growth and cell division, the envelope layers are enlarged by the combined actions of different protein machineries. Importantly, the co-ordination of this synthesis is vitally critical for maintaining the structural integrity of the envelope. Disturbed envelope biogenesis, thus, often leads to cell death, making it an excellent antibiotic target. The asymmetric nature of the OM lipid bilayer in Gram-negative bacteria provides a permeability barrier to both hydrophobic and large hydrophilic antimicrobials. This impermeability, in combination with efflux pumps, makes these organisms notoriously difficult to treat with antibiotics. Despite the critical importance of the Gram-negative cell envelope for developing effective antimicrobial therapies, genome-wide tools to dissect envelope biogenesis are still underdeveloped.
This post is offered on a fixed term basis until 28.02.2026
Please note that if you are successful to this role, you will require medical clearance before you can commence in the role.
As part of our commitment to career development for research colleagues, the University has developed 3 levels of research role profiles. These profiles set out firstly the generic competences and responsibilities expected of role holders at each level and secondly the general qualifications and experiences needed for entry at a particular level.
Key Accountabilities
Contribution to the research programme of the School/Institute under the clear guidance of a member of an academic staff or Principal Investigator
Use initiative and creativity to analyse and interpret research data and draw conclusions on the outcomes
Co-ordinate own work with that of others, deal with problems which may affect the achievement of research objectives and contribute to the planning of the project(s)
Present information on research progress and outcomes to a Principal Investigator or groups overseeing the research project
Write up results from own research activity and provide input into the research project’s dissemination, in whatever form (report, papers, chapters, book)
Work to deadlines and manage, with support, competing priorities
Ensure that personal knowledge in relevant fields of study is kept up to date
Use research resources (including, where required, laboratories, workshops and specialist equipment) as appropriate
Maintain academic standards and freedom, and work in accordance with university policies (e.g. equal opportunities, health and safety policies)
Possible supervision and demonstration duties within undergraduate laboratories and example classes or in teaching small seminar classes
Undertake relevant training and development activities to develop capacity for taking on wider responsibilities
Develop, with advice, an awareness of own professional development needs and a personal development strategy
The Person
Knowledge, Skills and Experience
Subject knowledge in microbiology
Good level of analytical skills and the ability to communicate complex information clearly, both orally and through the written word
The ability to work collaboratively with colleagues
The ability to use personal initiative and creativity to solve research problems
A strong background in genetic techniques for a range of bacterial species including mutagenesis, cloning of complex templates and development of reporter constructs
Significant knowledge and experience with bioinformatic techniques including annotation of sequences, genomic comparisons and homology searching of genes/proteins within publicly available databases
Experience in the development and optimization of laboratory methods with appropriate troubleshooting conditions
Desirable
Experience in chemical genomics approaches
Prior knowledge in working with secretion systems
Prior knowledge working with Staphylococcus aureus
Attributes and Behaviour
You should display analytical thinking and problem-solving skills towards molecular stopgaps and develop solutions towards assays
Have a collaborative mindset, both within the immediate laboratory and larger studies with other research groups
Be able to adapt, identifying gaps withi
