Research Fellow On Atomic Force Microscopy of
3 weeks ago
**Ref Number**
B04-03557
**Professional Expertise**
Research and Research Support
**Department**
UCL BEAMS (B04)
**Location**
London
**Working Pattern**
Full time
**Salary**
£39,508 - £47,355
**Contract Type**
Fixed-term
**Working Type**
Hybrid (20% - 80% on site)
**Available For Secondment**
No
**Closing Date**
15-May-2023
**About us**:
This postdoc position is related to a project funded by the UK Biotechnology and Biological Sciences Research Council, aiming to understand how broad-spectrum antibiotics target and kill Gram-negative bacteria. The London Centre for Nanotechnology (LCN) is an interdisciplinary enterprise between University College London, King’s College London and Imperial College London. In bringing together world-class infrastructure and leading nanotechnology research activities, the Centre aims to attain the critical mass to compete with the best facilities abroad. The LCN has strong relationships with the broader nanotechnology and commercial communities, and is involved in much major collaboration. As the world’s only such facility to be located in the heart of a metropolis, the LCN has superb access to corporate, investment and industrial partners. It is at the forefront of training in nanotechnology, and has a strong media presence aimed at educating the public and bringing transparency to this emerging science.
**About the role**:
The project will build on our recent progress in molecular-resolution atomic force microscopy on living E. coli, and will be complemented by NMR, electron microscopy and molecular dynamics simulations in Nottingham. Gram-negative bacteria are protected from their environment by a multi-layered bacterial cell envelope, consisting of an inner membrane, a cell wall, and an outer membrane. By targeting these membranes, antibiotics can provide broad-spectrum protection against such bacteria. Yet even for membrane-targetting antibiotics that have been used in the clinic for decades, we do not fully understand how they disrupt and/or permeate the bacterial cell envelope. Building on recent new insights and technological advances, this position is aimed to provide such understanding by using high-resolution atomic force microscopy on living bacteria, complementing assays using NMR, electron microscopy and molecular dynamics simulations. It offers an exciting opportunity to carry out research at the interface of microbiology and biophysics in a context that is most relevant for antimicrobial resistance. The position is initially funded for two years. The research will be carried out in Prof. Bart Hoogenboom’s biophysics research group at the London Centre for Nanotechnology, in close collaboration with Dr. Andrew Edwards’s and Dr Boyan Bonev’s microbiology labs at, respectively, Imperial College London and the University of Nottingham.
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