Scientist

Work Location IC: NCIStreet: 10 Center Dr Bldg: 10Room: 1B34A City: BethesdaState & Zip: MD 20814

Weekly Hours FT: 3040 hours per week

Overall Position Summary andObjectives

Contractor willindependently provide support services to satisfy the overalloperational objectives of the National Cancer Institute Center forCancer Research. The primary objective is to provide services anddeliverables through performance of support services.

Min Education Masters

Certifications & Licenses

• Ph.D. in Biology LifeSciences or a related discipline.
• Five yearsof specialized experience plus a Bachelors degree is equivalent toa Ph.D.
• Three years of specialized experienceplus a Masters degree is equivalent to a Ph.D.

Field of Study Miscellaneous Biology

Software Proficiency in the use of computers and computerprograms including expertise in Microsoft Office Suite (MS WordPowerPoint Excel) Powerpoint

Skills

• Research experience inimmunology and/or molecular biology
• Abilityto learn new techniques and teach known techniques to others.
• Knowledge of proper laboratory techniquesdisposal methods laboratory safety procedures and universalprecautions.
• Ability to work independently onprojects.
• Excellent analytical organizationalinterpersonal and time management skills.
• Strong communications skills both oral and written.
• Minimum of two (2) years of experience in alaboratory environment.
• Mouse handling andprocedures including intraperitoneal and intravenous injectionstumor measurements and dissections.
• ConfocalMicroscopy Immunologic studies including multiparameter flowcytometric processing and data analysis as well as in vitrofunctional assays including cell culture stimulation proliferationand cytokine production.
• Molecular studiestechniques including DNA and RNA extraction and quantification gelelectrophoresis western blotting PCR andimmunoprecipitation.

Statement of Work Details

• Conductlaboratorybased translational research with a focus on drugdevelopment for the treatment of cancer.
• Study cultured human and animal cell lines some of whichmay be derived from cancer tissue and analyze human and/or animaltissues with and without cancer.
• Perform awide range of sophisticated genetic biological and biochemicalassays including but not limited to DNA transfection of cellsmonolayer and anchorageindependent growth assaysimmunoprecipitation assays agarose gel electrophoresis and in vivoand in vitro kinase assays.
• Perform confocalimmuneconfocal microscopy of cells.
• Initiatedesign and develop new projects or assays that can enable theCenter for Cancer Research/Laboratory of U. Rudolf Rare TumorInitiative POB NCI to fulfill its responsibilities for advance incancer research and development of cancer diagnostics cancer drugsand cancer vaccines.

• Workwith staff on the interpretation of experiments performed byresearch scientists.
• Serve as an authority onthe tumor biology that is being studied and the assays that arebeing performed.
• Collaborate with NIH/NCIexpert staff to clarify needs and project objectives.
• Work with the scientific staff to define key questionsemerging from research projects in which experimental in vitro andin vivo analysis will contribute to elucidation of impact of novelexperimental therapeutics on immune cells cancer cells and tumorsgrown in mice in general mechanisms of cell transformationidentification of molecular targets and the development ofbiomarkers/biomarker signatures with potential to guide preventiondiagnosis or treatment.
• Perform studies thatresult in technical reports and information to advance cancerresearch.
• Participate in special committeespanels and colloquia that meet to determine policy or plan activityin the field.

• Interpretresults of initial analysis to reveal conclusions and shortcomings;identify pitfalls in the original choice of methods or experimentaldirection; redirect the study to achieve improvedresults.
• Prepare data sets Tables and Figuresfor collaborative papers prepared for publication in peerreviewedjournals; assemble and write papers for firstauthor publication inpeerreviewed papers.

• Identify the need for further investigations and/orpropose change in direction of work in light of accomplishmentsfindings and current and future mission objectives.

• Attend seminars within the National Institute ofHealth/Center for Cancer Research/Laboratory of U. Rudloff; pursueadditional training where necessary; participate in discussions atmeetings.
• Make oral presentations at variousconferences and seminars.

• Collaborate with scientists and statisticiansin NCI NIH and extramural research institutions as appropriate toadvance the research projects and elucidation of cancerrelateddata.

• Conductlaboratorybased translational research with a focus on drugdevelopment for the treatment of cancer.
• Studycultured human and animal cell lines some of which may be derivedfrom cancer tissue and analyze human and/or animal tissues with andwithout cancer.
• Collaborate with scientistsand statisticians in NCI NIH and extramural research institutionsas appropriate to advance the research projects and elucidation ofcancerrelated data.
• Perform confocalimmuneconfocal microscopy of cells.
• Performstudies that result in technical reports and information to advancecancer research.
• Collaborate with NIH/NCIexpert staff to clarify needs and project objectives.
• Serve as an authority on the tumor biology that is beingstudied and the assays that are being performed.
• Interpret results of initial analysis to revealconclusions and shortcomings; identify pitfalls in the originalchoice of methods or experimental direction; redirect the study toachieve improved results.
• Work with thescientific staff to define key questions emerging from researchprojects in which experimental in vitro and in vivo analysis willcontribute to elucidation of impact of novel experimentaltherapeutics on immune cells cancer cells and tumors grown in micein general mechanisms of cell transformation identification ofmolecular targets and the development of biomarkers/biomarkersignatures with potential to guide prevention diagnosis ortreatment.
• Participate in special committeespanels and colloquia that meet to determine policy or plan activityin the field.

• Perform a widerange of sophisticated genetic biological and biochemical assaysincluding but not limited to DNA transfection of cells monolayerand anchorageindependent growth assays immunoprecipitation assaysagarose gel electrophoresis and in vivo and in vitro kinaseassays.
• Prepare data sets Tables and Figuresfor collaborative papers prepared for publication in peerreviewedjournals; assemble and write papers for firstauthor publication inpeerreviewed papers.

Learns new laboratory techniques andprocedures

• Initiate designand develop new projects or assays that can enable the Center forCancer Research/Laboratory of U. Rudloff Rare Tumor Initiative POBNCI to fulfill its responsibilities for advance in cancer researchand development of cancer diagnostics cancer drugs and cancervaccines. Make oral presentations at various conferences andseminars.
• Identify the need for furtherinvestigations and/or propose change in direction of work in lightof accomplishments findings and current and future missionobjectives.
• Attend seminars within theNational Institute of Health/Center for Cancer Research/Laboratoryof U. Rudloff; pursue additional training where necessary;participate in discussions at meetings