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Phage Display Researcher
2 months ago
CK Group is partnering with Nxera Pharma UK Limited to recruit a Research Scientist/Senior Scientist position in the Protein Binder Discovery team based in Granta Park, Cambridge.
About the RoleWe are seeking an experienced phage display scientist to join the Protein Binders team, part of our Platform Technology division at Nxera Pharma. The successful candidate will use phage display expertise to identify protein binders against therapeutic membrane protein targets to enable structure determination and drug discovery efforts.
Key Responsibilities- Employ phage display technology to select protein binders against membrane protein targets
- Screen and validate hits using high-throughput assays, such as ELISA formats, BLI, and cell binding assays
- Subclone plasmid constructs for expression and protein engineering of VHH frameworks
- Biophysically evaluate VHH hits
- Share expertise and insights across the team, project, and department to influence project success
- PhD (or MSc/MRes with equivalent lab experience) in a relevant area of biology with at least 3 years of experience carrying out phage display campaigns
- Theoretical and practical expertise in phage display technologies and selection techniques
- Experience working with phage antibody libraries and preferably VHH display
- Strong molecular biology proficiency
- Demonstrable expertise in protein-protein interaction screening techniques
- Dynamic, analytical thinker with good problem-solving skills
- Strong interpersonal skills and effective interaction with scientists across disciplines
- Experience of measuring affinity using BLI or SPR
- Experience of affinity maturation
- Experience of library build (affinity maturation or immunised, naïve, or synthetic libraries)
- Experience in working with membrane proteins (particularly GPCRs)
- Computational expertise focused around protein binder design
Please note that applicants must hold entitlement to work in the UK. Please quote job reference 99230 in all correspondence.