Research Fellow in Ribosome Structure

2 weeks ago


Leeds, United Kingdom University of Leeds Full time

**Research Fellow in Ribosome Structure**:
**Are you an ambitious researcher looking for your next challenge? Do you have an established background in structural biology? Do you want to further your career in one of the UKs leading research intensive Universities?**

We are looking for an outstanding postdoctoral research fellow to join the Fontana and Calabrese labs at the University of Leeds. The fellow will be part of a large team (£5.7M, funded by a BBSRC Strategic Longer and Larger grant). The team aims to unravel the ‘ribosome code’, rules that explain how compositionally discting ribosomes enable translation of specific sets of mRNA. Specifically, the Research Fellow in Structural Biology will focus on the structural characterisation of the different ribosomes that we will purify during the project. This will include isolating specialised ribosomes from a variety of orgamisms, and structurally characterising these ribosomes by cryo-electron microscopy and structural mass spectrometry. This will be possible by taking advantage of the state-of-the-art equipment available at the Astbury Biostructure Laboratory (ABSL), which includes: two Titan Krios microscopes, equipped with Falcon 4 and Selectris Falcon 4 direct electron detectors; an Orbitrap Ultra-High Mass Range mass spectrometer (for native mass spectrometry of intact ribosomes); and a Orbitrap Eclipse mass spectrometer (for cross-linking mass spectrometry). We anticipate that by understanding the structure of different specialised ribosomes, by exploiting our combined cryo-EM and mass spectrometry toolkit, we will be able to dissect conserved mechanisms of ribosome specialisation across eukaryotes, allowing us to unravel the ‘ribosome code’.

You must have a PhD (or close to completition) in RNA, translation, structural biology or a closely allied discipline;and experience in either cryo-electron microscopy or mass spectrometry applied to the structural characterisation of macromolecules.

**To explore the post further or for any queries you may have, please contact**:
Dr Juan Fontana, Associate Professor.


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