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Research Associate

4 months ago

London, United Kingdom King's College London Full time

Job description

Investigation on RNA binding properties of disordered unconventional RNA binding motifs

Applications are invited for a postdoctoral scientist to investigate the mechanisms of RNA binding mediated by disordered regions lacking conventional RNA binding annotations in the laboratory of Professor Maria R (Sasi) Conte, Randall Centre for Cell and Molecular Biophysics, King’s College London.

ABOUT US

Our lab specialises in understanding the detailed mechanisms of interaction between proteins and RNA, with a major focus on the La-related proteins (LARPs). We use a combination of structural biology, biophysics and biochemistry techniques and work closely with interdisciplinary collaborators to test hypotheses in the context of cell biology and model organisms.

WE ARE LOOKING FOR

The successful applicant will find themselves working in a dynamic, interdisciplinary and exciting research environment and will be based in the Randall Centre on the Guy’s Campus, London, UK. The project will be supported by the state-of-the-art infrastructure of the Centre for Biomolecular Spectroscopy and the Randall Centre.

This post will be offered on an fixed-term contract until 3rd January 2025.

This is a full-time post - 100% full time equivalent

Key responsibilities
- Contribute to the research project as agreed with the principal investigators.
- Carry out molecular biology and protein biochemistry work as required by the project.
- Carry out integrated structural biology, biochemistry and biophysical work as required by the project.
- When required perform data acquisition and/or analyses at external locations ( _e.g_. various synchrotrons in UK and Europe, Crick Institute etc.)
- Maintain up-to-date, accurate and comprehensive laboratory notebooks and undertake data analysis.
- Prepare reports on results for written and verbal presentation to the principal investigator and for regular laboratory meetings.
- Work with the principal investigator to prepare manuscripts based on the work for publication.
- Follow the relevant research literature through a process of self-directed enquiry and continuing education.
- Adhere to any Safety procedures and Risk Assessment requirements and safe working practices.
- Contribute to the supervision and training of junior staff and students.
- Contribute to laboratory maintenance and administration (including health and safety issues, equipment maintenance, inventory control, ordering).
- Any other duties as agreed with the direct supervisor.
- The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post._

Skills, knowledge, and experience

Essential criteria

1. Experience in protein expression and purification for structural and biophysical studies

2. Experience in molecular biology techniques

3. Extensive experience in NMR spectroscopy of protein and RNA

4. Experience and background in protein-RNA interactions

5. Knowledge and competency in a wide range of biophysical techniques for macromolecular characterisation and interactions
_e.g._ CD, fluorescence spectroscopy, EMSA, titration calorimetry, mass spectrometry, SAXS, X-ray crystallography)

6. Self
- motivated, able to work independently and as part of a team

7. Excellent communication skills, both oral and written

Desirable criteria

1. Knowledge and experience in integrated structural biology

2. Knowledge and experience in intrinsically disordered proteins

3. Prior experience with SAXS/SANS

4. Ability to develop alternative technologies

Further information